Bacillus subtilis biofilm matrix components target seed oil bodies to promote growth and anti-fungal resistance in melon.

Beneficial microorganisms are used to stimulate the germination of seeds; however, their growth-promoting mechanisms remain largely unexplored. Bacillus subtilis is commonly found in association with different plant organs, providing protection against pathogens or stimulating plant growth. We report that application of B. subtilis to melon seeds results in genetic and physiological responses in seeds that alter the metabolic and developmental status in 5-d and 1-month-old plants upon germination. We analysed mutants in different components of the extracellular matrix of B. subtilis biofilms in interaction with seeds and found cooperation in bacterial colonization of seed storage tissues and growth promotion. Combining confocal microscopy with fluorogenic probes, we found that two specific components of the extracellular matrix, amyloid protein TasA and fengycin, differentially increased the concentrations of reactive oxygen species inside seeds. Further, using electron and fluorescence microscopy and metabolomics, we showed that both TasA and fengycin targeted the oil bodies in the seed endosperm, resulting in specific changes in lipid metabolism and accumulation of glutathione-related molecules. In turn, this results in two different plant growth developmental programmes: TasA and fengycin stimulate the development of radicles, and fengycin alone stimulate the growth of adult plants and resistance in the phylloplane to the fungus Botrytis cinerea. Understanding mechanisms of bacterial growth promotion will enable the design of bespoke growth promotion strains.

[Efficacy of lumbopelvic stabilization exercises in patients with lumbalgia]. / Eficacia de los ejercicios de estabilización lumbopélvica en pacientes con lumbalgia.

INTRODUCTION: Low back pain is one of the main causes of work disability in Mexico, conservative treatment includes performing exercises. OBJECTIVE: To assess the effects of lower back pain with a controlled exercise program to improve flexibility and strength in the core or center of the body. MATERIAL AND METHODS: We included 18 people, 12 women and six men between 24 and 70 years of age, with low back pain, with a score of 2 to 10 using the visual analogue scale (VAS), with or without referred pain in the lower limb. Participants were advised to refrain from taking medications, alcohol and intense physical activities during the treatment period. The participants carried out 12 sessions of 10 exercises in 3 sets of 10 repetitions, 3 times per week, evaluating: weight, distance toes, anterior rectum strength, abdominal diameter, initial and final Oswestry index. RESULTS: The results were analyzed with the Wilcoxon test, showing a decrease not only in pain (p 0.01), but an improvement in weight, flexibility and perception of disability due to the Oswestry scale (p 0.01) showing that the series of exercises proposed is an alternative to the management of low back pain. DISCUSSION: It is recommended the presence of a therapist who accompanies and corrects the postures so that the exercises are performed well, as well as giving value to the degree of disability caused by pain in the daily performance of the people who present it.

INTRODUCCIÓN: El dolor lumbar es una de las principales causas de incapacidad laboral en México; dentro del tratamiento conservador, se incluye la realización de ejercicios para la disminución del dolor. OBJETIVO: Valorar los efectos en la disminución del dolor lumbar con un programa controlado de ejercicios diseñados para mejorar la flexibilidad y fuerza en el núcleo o centro del cuerpo (core). MATERIAL Y MÉTODOS: Se incluyeron a 18 personas: 12 mujeres y seis hombres de entre 24 y 70 años con dolor lumbar, el cual estaba referido con una puntuación de 2 a 10 utilizando la escala visual análoga (EVA), con o sin dolor en la extremidad inferior. Se aconsejó a los participantes abstenerse de tomar medicamentos, alcohol y de realizar actividades físicas intensas durante el período de tratamiento. Los participantes realizaron 12 sesiones de 10 ejercicios en tres series de 10 repeticiones, tres veces por semana, evaluándose durante estas sesiones: peso, distancia dedos piso, fuerza de recto anterior, diámetro abdominal e índice de Oswestry inicial y final. RESULTADOS: Los datos se analizaron con la prueba de Wilcoxon. Se evidenció disminución no sólo en el dolor (p 0.01), sino una mejoría en el peso, la flexibilidad y la percepción de incapacidad por la escala de Oswestry (p 0.01), mostrando que la serie de ejercicios propuestos es una alternativa para al manejo del dolor lumbar. DISCUSIÓN: Se recomienda la presencia de un terapeuta que acompañe y corrija las posturas para que los ejercicios sean bien realizados, así como darle valor al grado de incapacidad que produce el dolor en el desempeño cotidiano de las personas que lo presentan.

Eficacia de los ejercicios de estabilización lumbopélvica en pacientes con lumbalgia / Efficacy of lumbopelvic stabilization exercises in patients with lumbalgia

Resumen: Introducción: El dolor lumbar es una de las principales causas de incapacidad laboral en México; dentro del tratamiento conservador, se incluye la realización de ejercicios para la disminución del dolor. Objetivo: Valorar los efectos en la disminución del dolor lumbar con un programa controlado de ejercicios diseñados para mejorar la flexibilidad y fuerza en el núcleo o centro del cuerpo (core). Material y métodos: Se incluyeron a 18 personas: 12 mujeres y seis hombres de entre 24 y 70 años con dolor lumbar, el cual estaba referido con una puntuación de 2 a 10 utilizando la escala visual análoga (EVA), con o sin dolor en la extremidad inferior. Se aconsejó a los participantes abstenerse de tomar medicamentos, alcohol y de realizar actividades físicas intensas durante el período de tratamiento. Los participantes realizaron 12 sesiones de 10 ejercicios en tres series de 10 repeticiones, tres veces por semana, evaluándose durante estas sesiones: peso, distancia dedos piso, fuerza de recto anterior, diámetro abdominal e índice de Oswestry inicial y final. Resultados: Los datos se analizaron con la prueba de Wilcoxon. Se evidenció disminución no sólo en el dolor (p < 0.01), sino una mejoría en el peso, la flexibilidad y la percepción de incapacidad por la escala de Oswestry (p < 0.01), mostrando que la serie de ejercicios propuestos es una alternativa para al manejo del dolor lumbar. Discusión: Se recomienda la presencia de un terapeuta que acompañe y corrija las posturas para que los ejercicios sean bien realizados, así como darle valor al grado de incapacidad que produce el dolor en el desempeño cotidiano de las personas que lo presentan.

Abstract: Introduction: Low back pain is one of the main causes of work disability in Mexico, conservative treatment includes performing exercises. Objective: To assess the effects of lower back pain with a controlled exercise program to improve flexibility and strength in the core or center of the body. Material and methods: We included 18 people, 12 women and six men between 24 and 70 years of age, with low back pain, with a score of 2 to 10 using the visual analogue scale (VAS), with or without referred pain in the lower limb. Participants were advised to refrain from taking medications, alcohol and intense physical activities during the treatment period. The participants carried out 12 sessions of 10 exercises in 3 sets of 10 repetitions, 3 times per week, evaluating: weight, distance toes, anterior rectum strength, abdominal diameter, initial and final Oswestry index. Results: The results were analyzed with the Wilcoxon test, showing a decrease not only in pain (p < 0.01), but an improvement in weight, flexibility and perception of disability due to the Oswestry scale (p < 0.01) showing that the series of exercises proposed is an alternative to the management of low back pain. Discussion: It is recommended the presence of a therapist who accompanies and corrects the postures so that the exercises are performed well, as well as giving value to the degree of disability caused by pain in the daily performance of the people who present it.

Age of Onset, Current Use of Alcohol, Tobacco or Marijuana and Current Polysubstance Use Among Male and Female Mexican Students.

AIMS: This study aimed to examine alcohol, tobacco or marijuana use onset and associations between age of onset and current use, and between age of onset and current polysubstance use among a sample of Mexican young people aged 17-20 years. METHODS: Alcohol, tobacco, marijuana and other substance use were identified in a 2012 cross-sectional survey of male and female students entering first year university in Mexico City. Chi-square tests and logistic regression models examined associations between gender, age of onset, order of onset, family substance use and current polysubstance use among 22,224 students. RESULTS: The largest proportions of males and females initiated alcohol at age 15, tobacco at ages 15-16 and marijuana at ages 16-17. Earlier alcohol and tobacco initiation was associated with past 30-day use of the same substance. Gender differences in alcohol and tobacco use were small but males were significantly more likely than females to use marijuana and be polysubstance users. Family substance use was associated with earlier onset. There was a significant association between the earliest age of any of the three substances and current polysubstance use. CONCLUSIONS: A number of contextual and cultural factors in Mexico may be contributing to early onset, continued use and polysubstance use and deserve attention in research and by prevention programs.

Parental monitoring and family relations: associations with drinking patterns among male and female Mexican students.

INTRODUCTION: Parental monitoring and family relations are recognized as protective factors for youth alcohol use. The purpose of this study was to investigate perceived parental monitoring and family relations among subgroups of Mexican youths with different patterns of drinking behaviors and consequences. METHODS: A latent profile analysis (LPA) identified profiles of drinking behavior in a cross-sectional survey of entering first year university students. Multinomial regression examined associations between parental monitoring, family relations and drinking profiles among 22,224 students. RESULTS: Both lower perceived parental monitoring and weaker perceived family relations were associated with heavier drinking profiles among males and females, but more strongly associated with female than male heavier drinking profiles. Being older, having parents with lower education, and not living with parents were also associated with lower parental monitoring and weaker family relations. There was a general trend of lower parental monitoring and weaker family relations as the profiles increased from Non/Infrequent-No Consequences to Excessive-Many Consequences Drinkers. Lower perceived parental monitoring and weaker perceived family relations were more strongly associated with drinking profiles among females than among males. Both the parental monitoring and family relations scales had similar associations with drinking profiles. CONCLUSIONS: Findings suggest that drinking norms and values may contribute to any protective influences of parental monitoring and family relations on Mexican youths' drinking. Research about changes in drinking norms, contextual factors, and youth-parent trust would inform the utility of parental monitoring or family relations as protective strategies against alcohol misuse among Mexican and Mexican American youths and also youths from other backgrounds.

Natural mentors and youth drinking: a qualitative study of Mexican youths.

Parental influences on youth drinking are well documented but not the influence of extended family members. This article explores extended family influences on alcohol use among Mexican youths and whether extended family members can be considered natural mentors. We conducted a qualitative study using ethnographic open ended interviews with 117 first year university students in Mexico City. The ethnographic interviews revealed six drinking groups: excessive, heavy, regular, occasional, abstainers and non drinkers. Youths reported close relationships with extended family members who provided counsel and acted as representatives of familial norms and values. The alcohol beliefs and behaviors of these family members, including their alcohol misuse, had a positive influence on youths' alcohol attitudes. The naturally occurring mentoring relationships of Mexican extended family members can positively influence moderate youth drinking. Natural mentoring relationships should be encouraged and facilitated in prevention efforts for Mexican youths, Mexican-American youths and potentially other Hispanic/Latino youths.

Alcohol Use among Mexican Youths: Is Familismo Protective for Moderate Drinking?

The construct of familismo has been identified as a potential protective cultural value in Hispanic cultures. This paper considers familismo and alcohol use among young people in Mexico. We conducted a qualitative study using ethnographic open ended interviews with 117 first year students at a large free public university in Mexico City between April-May 2011. The findings indicate that dimensions of familismo can act as protective factors against misuse of alcohol among Mexican youths and may be protective for moderate drinking. Future research should explore the relationship of familismo to gender roles and other cultural values during adolescence. Given the enduring influence of Mexican cultural values among Mexican Americans the research has implications for prevention programs for both Mexican and Mexican American youths.

Drinking patterns and victimization among male and female students in Mexico.

AIMS: The purpose of this study is to estimate the prevalence of alcohol use and alcohol-related consequences, identify drinking profiles using latent profile analysis (LPA), and investigate associations between profiles and violent victimization among young people in Mexico. METHODS: LPA identified profiles of drinking behavior in a survey of entering first year university students. Multinomial and logistic regression examined associations between drinking patterns, socio-demographic variables and violent victimization. RESULTS: The LPA identified five profiles of behaviors and consequences among the 22,224 current, former and never drinkers: Non/Infrequent-No Consequences, Occasional-Few Consequences, Regular-Some Consequences, Heavy-Many Consequences and Excessive-Many Consequences drinkers. The Occasional-Few Consequences profile comprised the largest, and the Excessive-Many Consequences profile the smallest, group of drinkers. Multinomial regression showed males and older students more likely to be Heavy or Excessive-Many Consequences drinkers. Living alone was associated with higher odds, and higher maternal education with lower odds, of being a Non/Infrequent-No Consequences drinker. Heavier drinking profiles were more likely to experience violent victimization adverse consequences. Logistic regression showed male and female Heavy and Excessive-Many Consequences drinkers had the highest odds, and Non/Infrequent drinkers the lowest odds, of experiencing any victimization. CONCLUSION: Findings suggest changes in male and female drinking behavior and a continuation of the established pattern of infrequent but high consumption among Mexican youths. Both male and female Heavy and Excessive-Many Consequences drinkers were at elevated risk for experiencing victimization. Identifying cultural gender norms about drinking including drinker expectations and drinking context that contribute to these patterns can inform prevention efforts.

Changes in alcohol use among first year university students in Mexico.

BACKGROUND: Alcohol use and misuse is widespread among university students in many countries. Specific cultural factors may impact alcohol use after entering university. OBJECTIVE: This paper considers changes in alcohol use among first year university students in Mexico. METHODS: A qualitative study using ethnographic interviews with 57 female and 60 male student drinkers in Mexico City from March to June 2011. Each interview was evaluated by a set of thematic codes developed inductively from the interviews. Findings from excessive, heavy, regular, occasional drinkers, abstainers, and non-drinkers were analyzed to explore whether or not linkages existed between and/or among particular themes. RESULTS: Students reported factors associated with changes in role and status, friendships, and increased autonomy as reasons for increasing or decreasing their alcohol use after entering university. CONCLUSIONS/IMPORTANCE: Understanding the influence of Mexican cultural norms on alcohol use among Mexican and Mexican Americans can be helpful in informing studies and preventive efforts among both Mexican and Mexican American young people.

Ras regulates kinesin 13 family members to control cell migration pathways in transformed human bronchial epithelial cells.

We show that expression of the microtubule depolymerizing kinesin KIF2C is induced by transformation of immortalized human bronchial epithelial cells (HBEC) by expression of K-Ras(G12V) and knockdown of p53. Further investigation demonstrates that this is due to the K-Ras/ERK1/2 MAPK pathway, as loss of p53 had little effect on KIF2C expression. In addition to KIF2C, we also found that the related kinesin KIF2A is modestly upregulated in this model system; both proteins are expressed more highly in many lung cancer cell lines compared to normal tissue. As a consequence of their depolymerizing activity, these kinesins increase dynamic instability of microtubules. Depletion of either of these kinesins impairs the ability of cells transformed with mutant K-Ras to migrate and invade matrigel. However, depletion of these kinesins does not reverse the epithelial to mesenchymal transition (EMT) caused by mutant K-Ras. Our studies indicate that increased expression of microtubule destabilizing factors can occur during oncogenesis to support enhanced migration and invasion of tumor cells.

Parental monitoring and alcohol use among Mexican students.

Parental monitoring has been described as a protective factor and useful strategy to prevent substance misuse among youths. The aim of this study was to examine whether perceived parental monitoring influences frequency of alcohol use, age of drinking onset and risky drinking among entering public high school and university students in Mexico City. The study is a cross-sectional survey of entering first year students in the high school and university school system of a large public university in Mexico City conducted during registration at the beginning of the school year. In 2008, of 34,840 students accepted to the affiliated high schools, 28,996 students (51.8% female) completed the alcohol survey and of 37,683 students accepted into university 30,084 students (51.5% female) completed the alcohol survey. The findings suggest that compared to students with higher perceived parental monitoring those reporting lower perceived parental monitoring were more likely to report risky behavior. They were more likely to be ever drinkers, frequent drinkers, have earlier age of onset and high AUDIT scores. Overall, higher parental monitoring was strongly associated with being female and lower parental monitoring with being male. Our findings suggest that more research on parental monitoring as a protective strategy against alcohol misuse is needed. Research focusing on cultural factors including gender and age-related norms and familismo would increase knowledge of the association of parental monitoring and alcohol use among Mexican youths, Mexican American youths and potentially youths from other Hispanic backgrounds.

El consumo riesgoso y dañino de alcohol y sus factores predictivos en adolescentes estudiantes del bachillerato / Hazardous and harmful drinking among Mexican adolescents: prevalence and predictive factors

Alcohol is commonly used among adolescents in Mexico. In a representative survey of Mexico's general population, 30% of the teenagers reported current drinking, whereas 8% incurred in binge drinking (5 or more drinks per occasion) at least once during the last year. In addition, 3% reported three or more alcohol dependence symptoms over the last 12 months. In this Mexican age group, alcohol consumption importantly contributes to the most frequent causes of morbidity and mortality (e.g. accidents, violence, homicides, suicide and risky behaviors). Data from a representative survey of adolescents attending middle and high school in Mexico City suggests that adolescents attending Mexico's schools run a high risk for alcohol problems. In this study, prevalence of lifetime and current drinking, and of current binge drinking (5 or more drinks per occasion) were, respectively, 65.8%, 35.2%, and 23.8%. These rates are substantially higher than those described in teenagers from Mexico's general population. Furthermore, although among Mexico's general population the prevalence of drinking has traditionally been higher among male than female adolescents, no gender differences in prevalence of alcohol consumption have been reported among Mexican middle and high school students. This suggests that female adolescents in Mexico's schools have become equally exposed to drinking as their male counterparts. Despite these trends, there is a paucity of studies examining drinking patterns and their respective correlates among Mexican adolescents attending post-elementary education. To our knowledge, in this population, there are no published prevalence estimates of Hazardous and Harmful Drinking (HHD). HHD is defined as a pattern of alcohol consumption conferring to the individual a greater risk for health problems, or frankly conducive to medical or psychological complications (e.g. accidents, victimization, violence, alcohol dependence, liver cirrhosis and/or other medical complications). The Alcohol Use Disorders Identification Test (AUDIT), developed by the World Health Organization, is currently the only instrument specifically designed to identify HHD. Although the AUDIT was initially validated only among adult patients in primary care settings, this instrument has consistently shown to be valid and reliable in detecting alcohol problems in different populations, such as the adolescent population in many countries around the world. Given the public health implications of estimating the frequency of HHD among adolescents attending high school in Mexico, and given the importance of elucidating the variables influencing this problem, we decided to conduct the analysis presented here. To our knowledge, this is the first report published in the international literature on the prevalence of HHD among adolescents attending high school in a Latin American country. Objective In the study described here, derived from a Universidad Nacional Autónoma de México (UNAM) project entitled <>, we aimed at examining the frequency and risk factors for HHD among Mexican high school students. More specifically, our objectives were: 1. to determine the past-year prevalence of HHD among high school freshmen; and 2. to examine, in this population, the effects of demographic and family variables in the likelihood of HHD. Subjects and methods This study was a cross-sectional survey conducted at the beginning of the school year during the registration period between September 1st and September 30th, 2005. In 2005, a total of 34000 students were accepted to initiate college at the nine UNAM college campuses located in the Mexico City metropolitan area. Of these, 28784 students (87.4 %) (age=14.8 [±2.5] years; 51.0% women) consented in answering the survey and provided complete data. Consequently, 521 6 students (12.6%) were excluded from the analysis due to a lack of consent, incomplete data or their absence at the time of registration. We used the AUDIT to examine past-year prevalence of HHD. This self-report instrument includes 10 items that examine frequency and intensity of drinking (items 1-3), presence of alcohol dependence symptoms (items 4-6), and negative consequences of drinking (items 7-10), yielding a maximum possible score of 40 points. Among adult patients in primary care settings, it has been accepted that an AUDIT score of 0-7 points reflects safe levels of alcohol consumption, whereas a score of 8 points or higher indicates the presence of Hazardous and/or Harmful Drinking. It has been described however, that among adolescents, an AUDIT score of 3 points or higher reliably identifies those students experiencing this problem. In the analysis presented here, we separately examined and reported prevalence estimates and correlates for HHD using both AUDIT's cut-off scores (≥3 and ≥8). The AUDIT was administered at the same time that a wellness screening survey that the UNAM Medical Services routinely administer to all registering high school freshmen at the beginning of the school year. Questions in the wellness survey pertained students' medical and dental health, family medical history, immunizations, and use of tobacco and other drugs. In addition, demographic and socioeconomic information was obtained from a questionnaire also routinely administered by the UNAM registrar's office. This questionnaire included 37 items inquiring about gender, age, employment and marital status, monthly family income, parental education, place and type of residency, persons with whom the student resided, and questions on previous academic performance. We estimated the prevalence of HHD and their respective 95% confidence intervals (95% CI) in the total of the sample, and separately by age group, gender, working status, monthly family income, parental education, and by variables reflecting whether the students lived with their family, peers, or alone. These variables were modeled using simulated binary terms (0, 1). Subsequently, a multinomial logistic regression was used to examine the relationship between HHD and the demographic and socioeconomic variables listed above. Variables were entered simultaneously into the logistic regression equation. To summarize the level of risk of HHD conferred by significant variables in the logistic regression model, odds ratios (OR) and their respective 95% CI's were estimated. All the significant effects reported here were adjusted considering the effects of the remaining demographic and socioeconomic variables. Results Among the high school students examined here, the prevalence of HHD was 4.0% when an AUDIT cut-off score of ≥ 8 was used. When an AUDIT score of ≥3 was considered, a frequency of 1 7.2% was observed. Men (AUDIT ≥8: 5.4%; AUDIT ≥3: 21.4%) experienced this problem more frequently than women (AUDIT≥8: 2.6%; AUDIT≥3: 13.1%). The highest prevalence of HHD among men was observed in all age groups and regardless of working status, family income, parents' education, or regardless of the persons with whom the student reported to live with. Controlling for demographic and socioeconomic differences between men and women, we found that the risk of experiencing HHD among men was approximately two times higher than among women (OR's and 95% CI's for AUDIT's cut-off score ≥8 or ≥3, respectively 2.0[1.6-2.4] and 1.6[1.5-1.8]).

De acuerdo a la Encuesta Nacional de Adicciones (ENA) (Medina-Mora et al., 2003), de 1990 al 2002, la prevalencia en el consumo de alcohol durante los últimos 12 meses entre los jóvenes mexicanos de 12 a 17 años de edad, se elevó del 27.6% al 30%. De manera más reciente, la Encuesta de Estudiantes de Nivel Medio y Medio Superior de la Ciudad de México (EENMMS) (Villatoro et al., 2003), describió que el consumo alguna vez en la vida y el consumo durante el último mes de bebidas etílicas afectó respectivamente al 65.8% y al 35.2% de los estudiantes. Llama la atención que la frecuencia del consumo alguna vez en la vida reportada por la EENMMS es considerablemente mayor que la descrita en los adolescentes de la población general urbana (39.8%) por la ENA. Además, las diferencias de género reportadas por la ENA, en las que los hombres tradicionalmente beben con más frecuencia que las mujeres, no fueron encontradas por la EENMMS en la población estudiantil del nivel medio y medio superior. Hasta lo que nosotros sabemos, se desconoce cuál es la prevalencia en esta población de patrones de consumo problemático de alcohol como serían el consumo riesgoso y dañino (CRDA) o el consumo dependiente. Por lo que se desconoce si existen diferencias entre la población estudiantil y los adolescentes de la población general, en la prevalencia de estos problemas. El CRDA se define como un patrón de consumo de bebidas embriagantes, que se sitúa en un continuum de severidad, que coloca al sujeto en riesgo de desarrollar problemas de salud y/o que puede desembocar en francas complicaciones físicas y/o psicológicas (accidentes, victimización, violencia, dependencia al alcohol, etc.). Objetivo En el estudio que se presenta aquí, nos propusimos: 1) estimar la prevalencia durante el último año del CRDA en los estudiantes de primer ingreso al nivel bachillerato de la UNAM, y 2) evaluar la influencia de las variables sociodemográficas y familiares en el riesgo para el CRDA. Material y métodos El diseño del estudio fue el de una encuesta transversal en los estudiantes del primer año del bachillerato en el sistema escolarizado de la UNAM. Se estudiaron a 28 784 estudiantes (87.4 % de la población total). Se utilizó el Alcohol Use Disorders Identification Test (AUDIT) -versión en español para detectar el CRDA en la población adolescente. En este análisis empleamos tanto los puntos de corte recomendados para evaluar el CRDA en adultos (AUDIT≥8) como el puntaje recomendado para la población adolescente (AUDIT≥3). Se utilizaron porcentajes, promedios y desviaciones estándar para el análisis de las variables demográficas, y pruebas de contraste de medias (análisis de varianza) y de proporciones ( χ²) de acuerdo a la variable. Se utilizó la regresión logística multinomial para examinar la asociación de las variables demográficas y familiares con el CRDA. Se calcularon los odds ratios (OR) con intervalo de confianza al 95% para resumir el nivel de riesgo de ser afectado por el CRDA. Resultados Cuando se usó la definición del CRDA para la población adulta, se encontró que 4.0% de los estudiantes lo presentaron, frente a 1 7.2% cuando se utilizó el puntaje del AUDIT recomendado para los adolescentes. El riesgo de experimentar el CRDA fue casi dos veces mayor en los hombres que en las mujeres.

Prevalencia del consumo riesgoso y dañino de alcohol y factores de riesgo en estudiantes universitarios de primer ingreso / Prevalence of risky and harmful alcohol consumption and risk factors in freshmen students

Background In Mexico, alcohol is the most widely used substance among young adults. Alcohol consumption in this age group contributes importantly to the most frequent causes of mortality and morbidity (e.g., accidents, violence, homicides, suicide and risky behaviors). Around the world, college or university attendance has emerged in the literature as a risk factor for drinking problems among young adults. In Mexico, data from the most recent National Survey on Addictions showed that lifetime and current drinking is experienced by more than half of the Mexicans attending college education. Despite this, in our country there is a paucity of epidemiological studies examining drinking behavior and correlates among those attending college. Findings in non-representative samples of students attending public and private universities in Mexico City suggest that, during the last two decades, there has been an increase in the frequency of lifetime and current drinking in this population. Additionally, these studies have shown that, in comparison to young adults of the same age in the general population, university students may experience a greater prevalence of lifetime and current alcohol drinking. Regarding the frequency of unhealthy drinking among Mexican college students, to our knowledge there are no prevalence estimates of hazardous or harmful drinking published. However, observations in non-random samples of university students in Mexico City suggested that at least one in three men and one in five women incurred in unhealthy drinking (e.g., ≥ 5 drinks per occasion or drinking to intoxication) at least once during the last month. Hazardous and harmful drinking is respectively defined by a pattern of alcohol consumption conferring a greater risk for health problems or that is frankly conducive to medical or psychological complications (e.g., accidents, victimization, violence, alcohol dependence, liver cirrhosis and/or other medical complications). The Alcohol Use Disorders Identification Test (AUDIT), developed by the World Health Organization, is currently the only instrument specifically designed to identify hazardous and harmful drinking. Although the AUDIT was initially validated among older adult patients in primary care settings, this instrument has consistently shown to be valid and reliable in detecting alcohol problems in different populations such as the college students in many countries around the world. Given the public health implications of estimating the frequency of hazardous and harmful drinking among college students in Mexico, and given the importance of elucidating the variables influencing this problem, we decided to conduct the present study. To our knowledge, this is the first report published in the international literature on the prevalence of hazardous and harmful drinking among college students in a Latin American country. Objective In the analysis described here, derived from the project entitled Early Identification and Treatment of Problem Drinkers at the National Autonomous University of Mexico (UNAM), our aim was to examine the frequency and risk factors for hazardous and harmful drinking among Mexican university students. More specifically, our objectives were: 1. To determine the past-year prevalence of hazardous and harmful drinking among UNAM college freshmen; and 2. To examine in this population the effects of demographic and family variables on the likelihood of hazardous and harmful drinking. Subjects and methods This study was a cross-sectional survey that was conducted at the beginning of the school year during the registration period between September 1st and September 30th, 2005. In 2005, a total of 34 000 students were accepted to initiate college at the nine UNAM college campuses located in the Mexico City metropolitan area. Of these, 24 921 (73.3%) students (age=18.7±4.3 years; 55.7% women) consented in answering the survey and provided complete data. Consequently, 9 079 students (26.7%) were excluded from the analysis due to lack of consent, incomplete data or due to their absence at the time of registration. We used the Alcohol Use Disorders Identification Test (AUDIT) to examine past-year prevalence of hazardous and harmful drinking. This self-report instrument includes 10 items that examine frequency and intensity of drinking (items 1-3), presence of alcohol dependence symptoms (items 4-6) and negative consequences of drinking (items 7-10), yielding a maximum possible score of 40 points. Among adult patients in primary care settings, it has been accepted that an AUDIT score of 0-7 points reflects safe levels of alcohol consumption, whereas a score of 8 points or greater indicates the presence of hazardous and harmful drinking. It has been described, however, that among college students, an AUDIT score of 6 points or greater reliably identifies those students experiencing this problem. In the analysis presented here, we separately examined and reported the prevalence estimates and correlates of hazardous and harmful drinking using both AUDIT cut-off scores (≥ 6 and ≥ 8). The AUDIT was administered at the same time as a wellness screening survey that the UNAM Medical Services routinely administer to all registering freshmen at the beginning of the school year. Questions in the wellness survey pertained students' medical and dental health, family medical history, immunizations, use of tobacco and other drugs. In addition, demographic and socioeconomic information was obtained from a questionnaire also routinely administered by the UNAM registrar's office. This questionnaire included 37 items inquiring about gender, age, employment and marital status, monthly family income, parental education, place and type of residency, persons with whom the student resided, and questions on previous academic performance.

Antecedentes En México, el alcohol es la sustancia potencialmente adictiva que se utiliza con mayor frecuencia por los adultos jóvenes. Información proveniente de la Encuesta Nacional de Adicciones más reciente muestra que más de 50% de los jóvenes entre los 18-29 años ha consumido bebidas alcohólicas al menos una vez durante el último mes. En la Ciudad de México se ha encontrado que más de la mitad de las mujeres y cerca de dos terceras partes de los hombres entre 18-29 años de edad consume regularmente bebidas alcohólicas. Durante los últimos años, el consumo de bebidas alcohólicas se ha venido incrementando importantemente entre los jóvenes mexicanos de ambos sexos en edad de recibir una educación superior. A nivel internacional, la bibliografía sugiere que la población estudiantil de los centros de educación superior es un grupo de mayor riesgo para el desarrollo de problemas por consumo de alcohol. En México, aunque se desconoce si los estudiantes de educación superior son un grupo de mayor riesgo para estos abusos, algunas encuestas y reportes sugieren que los problemas por consumo de alcohol tienen una importancia creciente. En cuanto al consumo de alcohol que excede los niveles seguros para la salud (≥2 bebidas estándar al día en las mujeres o ≥3 bebidas estándar al día en los hombres), el Observatorio Mexicano del Alcohol y Drogas describió que en el año 2002 el consumo de cinco o más copas por ocasión de consumo afecta a tres de cinco hombres y a una de cinco mujeres. Aunque problemas metodológicos y sesgos de selección potenciales en estas encuestas dificultan su interpretación, sus resultados sugieren que el consumo de alcohol, particularmente el consumo riesgoso y potencialmente dañino, es común entre los estudiantes universitarios de la Ciudad de México. El consumo riesgoso y dañino de alcohol (CRDA) se sitúa en un continuum de severidad y se define como un patrón de consumo de bebidas embriagantes que colocan al sujeto en riesgo de desarrollar problemas de salud y/o que desemboca en francas complicaciones físicas y/o psicológicas (accidentes, victimización, violencia, dependencia al alcohol, cirrosis hepática, etc.). De acuerdo a los reportes de la bibliografía internacional, este es el primer estudio publicado sobre la prevalencia de consumo peligroso y dañino de alcohol en estudiantes universitarios en América Latina. Objetivo En el trabajo que se presenta aquí, que forma parte del proyecto para la Identificación Temprana y Tratamiento Oportuno de bebedores con Consumo Excesivo de Alcohol en Estudiantes Universitarios de la UNAM, nos propusimos evaluar la prevalencia del CRDA durante el último año y examinar los factores de riesgo y protección respectivos en estudiantes de primer ingreso a la licenciatura de la Universidad Nacional Autónoma de México. De manera especifica, nos propusimos: 1) estimar la prevalencia del CRDA durante el último año en los estudiantes de primer ingreso a la licenciatura de la UNAM, y 2) evaluar en esta población la influencia de las variables sociodemográficas y familiares en el riesgo para el CRDA. Material y métodos Se trató de un estudio transversal en el que se estudiaron 24921 estudiantes del primer año de la licenciatura de la UNAM (edad=18.7±4.3 años; 55% mujeres). Para detectar aquellos estudiantes que en el último año incurrieron en el CRDA, se utilizó el instrumento de tamizaje Alcohol Use Disorder Identification Test (AUDIT). Se utilizó la regresión logística multinomial para examinar los efectos de las variables demográficas y sociofamiliares, así como para calcular Odds Ratios (OR) y sus respectivos intervalos de confianza al 95%. Este instrumento consiste de 10 preguntas que exploran la frecuencia e intensidad del consumo de bebidas alcohólicas. Con el objetivo de poder comparar nuestros hallazgos con los de otros investigadores, se examinaron y se reportan separadamente las prevalencias del CRDA con base en puntos de corte de 8 y de 6 en el AUDIT. Para el reporte de datos demográficos y puntajes del AUDIT, se utilizaron porcentajes, promedios y desviaciones estándar. Se emplearon las pruebas de contraste de medias (análisis de varianza) y de proporciones (χ2) dependiendo de la naturaleza de cada variable. Se calcularon las prevalencias del CRDA con sus respectivos intervalos de confianza al 95%. Resultados Usando un puntaje de corte en el AUDIT de ocho y de seis puntos, la prevalencia del CRDA durante el último año fue respectivamente de 11.1% y de 18.4%. Esta fue mayor en los hombres (AUDIT≥8: 17.3%; AUDIT≥6: 27.4%) que en las mujeres (AUDIT≥8: 6.2%; AUDIT≥6: 11.3%). Además del sexo masculino, aquellos estudiantes que trabajaban y que reportaron un mayor ingreso familiar mensual, tuvieron un mayor riesgo de experimentar el CRDA. En las mujeres, pero no en los hombres, un mayor nivel educativo tanto en el padre como en la madre también se relacionó con un incremento en el CRDA. Contrariamente, una mayor edad y el ser casado se asoció con una reducción en el riesgo del CRDA.

Prevalence and associated factors for episodic and chronic daily headache in the Colombian population.

There are multiple risk factors for chronic daily headache (CDH), but they are usually assessed in an isolated form without an adequate control for confounders. CDH is considered a variant of episodic headache, but studies have not gathered enough evidence to evaluate simultaneously CDH and episodic in the same population. We set out to establish simultaneously the factors associated with chronic daily or episodic headache in a population setting, using a cross-sectional survey in a random sample of 1505 adult urban inhabitants (Bucaramanga, Colombia). The survey asked questions about headache, family and personal history of disease, and consumption or abuse of caffeine, alcohol, hypnotics and analgesics. The association among independent variables and CDH or episodic headache was made with multinomial logistic regression. Female gender, arterial hypertension or cranial trauma history, and a high score in the depression scale are associated with episodic headache and CDH. Parents with CDH, the complaint of multiple arousals during sleep and use of hypnotics are associated with CDH, but not with episodic headache. Age <36 years, alcoholism and snoring are factors associated only with episodic headache. Chronic daily headache and episodic headache have several common risk factors, but there are other factors not shared by both conditions.

[Validation of the SCOFF questionnaire for screening the eating behaviour disorders of adolescents in school]. / Validación del cuestionario SCOFF para el cribado de los trastornos del comportamiento alimentario en adolescentes escolarizadas.

OBJECTIVE: To determine the internal consistency and criteria validity of the SCOFF questionnaire for screening adolescent eating behaviour disorders. DESIGN: Validation study with stochastic sampling. SETTING: Three schools at Bucaramanga, Colombia. PARTICIPANTS: 241 students selected at random from 1595 possible ones. MAIN INTERVENTIONS: SCOFF questionnaire and semi-structured CIDI interview, administered independently. MAIN MEASUREMENTS: Social and personal details, Cronbach's alpha, sensitivity analysis, specificity, forecast values, Cohen mean kappa test, and ROC curve. RESULTS: Sensitivity found was 81.9% (95% CI, 70.7-89.7) and specificity, 78.7% (95% CI, 71.7-84.6). The area under the ROC curve was 0.8596 (95% CI, 0.8108-0.9084); the Cronbach's alpha test, 0.436, and the Cohen mean kappa, 0.554 (95% CI, 0.425-0.683). CONCLUSIONS: The SCOFF questionnaire is an acceptable alternative for screening groups of adolescents because of its simplicity and speed of administration.

Validation of a migraine screening questionnaire in a Colombian university population.

In studies of migraine prevalence, it is important to be aware of the discriminative capacity of the test used. We set out to validate a Spanish translation of Michel's Standardized Migraine Diagnosis Questionnaire. The questionnaire was applied on all active students of the School of Psychology of the Universidad Autónoma de Bucaramanga, Colombia. A neurologist interviewed a random sample to substantiate the diagnosis of migraine. Cronbach's alpha was calculated and factors analysis was made in order to estimate internal consistency, test-retest to find reproducibility, and ROC curve and diagnostic indicators were calculated to estimate the criteria validity. Of 357 students that answered the questionnaire, 188 (52.7%) were selected at random to attend an appointment with a neurologist, but only 170 had this interview. Cronbach's alpha on these 357 questionnaires is 0.7497. All the questions on the questionnaire represented two main factors. This test had sensitivity in 37.9% [95% confidence interval (CI) 25.8, 51.7], specificity in 99.1% (95% CI 94.4, 100), positive predictive value in 95.7% (95% CI 76.0, 99.8), and negative predictive value in 75.5% (95% CI 67.6, 82.1) for 17 or more points. The area below ROC curve is 0.8529 (95% CI 0.8035, 0.9217). Among 84 students who took the questionnaire a second time, the average score on the first survey was 12.33 +/- 7.46 points, while the average score on the second take was 11.26 +/- 7.85 (P = 0.069). Agreement for migraine is 83.3% (95% CI 73.6, 90.6; Cohen's kappa = 0.6650 +/- 0.1061). The Spanish translation of Michel's Questionnaire is easy to answer and has good internal consistency, but its reproducibility and sensibility are modest; however, the ROC curve is acceptable to discriminate migraine patients from normal subjects.

Evaluación de un programa de rehabilitación del paciente esquizofrénico / Evaluation of a rehabilitation program for schizophrenics

Se trata de un estudio quasi-experimental para la rehabilitación de un grupo de pacientes psiquiátricos de consulta externa con diagnóstico principal de esquizofrenia. Los objetivos generales del proyecto fueron mantener la estabilidad en el cuadro clínico y mejorar el funcionamiento psicosocial y el desempeño cotidiano de los pacientes. El programa tuvo una duración de 14 meses. Se trabajó con un diseño quasi-experimental de pretest-postest con un grupo control (N = 17) y uno experimental (N = 21). El grupo control recibió la atención psiquiátrica habitual y el grupo experimental además de recibir ésta, asistió a un programa de intervenciones múltiples que incluían psicoterapia individual y de grupo, dinámica familiar con los parientes responsables de cada paciente, intervención psicosicial y terapia de actividades que incluían terapia recreativa, expresión corporal musicoterapia y taller de dibujo artístico. Los pacientes fueron evaluados con la Cédula de Entrevista Diagnóstica Internacional (CIDI) (Robins, 1988) con la Escala de Síntomas positivos y negativos (PANSS) (Andrease, 1984), con la Escala de Síntomas Extrapiramidales de DiMascio (Chovinard, 1980), y con la Escala de Desempeño Cotidiano desarrollada en el Instituto Mexicano de Psiquiatría (De la Fuente y Díaz, 1993). Así mismo, se evaluó al familiar responsable con la CIDI. En el 30.5 por ciento de los familiares hubo también patología: el diagnóstico más frecuente fue el de trastorno afectivo con 16.6 por ciento, en segundo lugar los trastornos fóbicos y/o somatoformes con 11.1 por ciento. El trastorno psiquátrico se asoció con algún otro tipo de trastorno o emocional (diagnosticado por el médico) en el 13.8 por ciento de la muestra. Los pacientes del grupo experimental mostraron un mejor rendimiento psicosocial en la evluación con la Escala de Desempeño cotidiano; en particular, en la sintomatología psiquiátrica y en las relaciones interpersonales. La evaluación final del proyecto permite concluir que este demostró ser de gran utilidad en áreas específicas de la vida de los pacientes y logró disminuir una proporción importante de las discapacidades presentes así como el aprovechamiento de las funciones conservadas

El manejo integral del paciente esquizofrénico. Un modelo del Instituto Mexicano de Psiquiatría / The integral management o the schizophrenic patient. A model of the Mexican Institute of Psychiatry

Este artículo describe los resultados preliminares del proyecto desarrollado en el Instituto Mexicano de Psiquiatría para el Tratamiento y Rehabilitación del Paciente Esquizofrénico de consulta externa. Los objetivos generales del proyecto fueron mantener la estabilidad en el cuadro clínico y mejorar el funcionamiento psicosocial y el desempeño cotidiano de los pacientes. El programa tuvo un duración de 14 meses. Se trabajó con un diseño quasi-experimental de pretest-postest con un grupo control (N=17) y uno experimental (N=21). El grupo control recibió la atención psiquiátrica habitual y el grupo experimental además de recibir ésta, asistió a un programa de intervenciones múltiples que incluían psicoterapia individual y de grupo, dinámica familiar con los familiares responsables de cada paciente, intervención psicosocial y terapias de actividades que incluían terapia recreativa, expresión corporal, musicoterapia y taller de dibujo artístico. En este trabajo se reportan los resultados del proyecto genral. Los resultados de cada una de las intervenciones terapeúticas se publicarán en otros trabajos. Los pacientes fueron evaluados con la Cédula de Entrevista Diagnóstica Internacional (CIDI) (Robins, 1988), con la Escala de Síntomas Positivos y negativos (PANSS) (Andreasen, 1984), con la Escala de Síntomas Extrapiramidales de DiMascio (CHovinard, 1980), y con la Escala de Desempeño Cotidiano desarrollada en el Instituto Mexicano de Psiquiatría (De la Fuente y Díaz, 1993): Así mismo, se evaluó al familiar responsable con CIDI. Los pacientes del grupo experimental mostraron un mejor rendimiento psicosocial, en relación al grupo control en la evaluación con la Escala de Desempeño Cotidiano, en particular, en la sintomatología psiquiátrica y en las relaciones interpersonales. La evaluación final del proyecto para el manejo integral del paciente esquizofrénico, en el área de la rehabilitación psicosocial, nos permite concluir que éste mostró ser de gran utilidad en áreas específicas de la vida de los pacientes, logrando disminuir una proporción importante de las discapacidades presentes así como el aprovechamiento de las funciones conservadas. Los resultados de cada uno de los estudios realizados y las intervenciones terapéuticas serán reportados en otras publicaciones